African swine fever PCR detector

Product Overview

The HM-P16 African swine fever PCR detector is mainly used to run real-time fluorescence quantitative PCR experiments. Through fluorescence excitation and collection, the experimental process is monitored in real time, and the experimental data is plotted into fluorescence curves, which are presented in real time on the instrument display interface. The experimental data is fitted and analyzed, and finally a PDF format experimental report can be generated.

Fast and accurate qualitative and quantitative analysis of target nucleic acids contained in biological samples taken from other animals (such as blood, body fluids, etc.), as well as standard curve, melting curve, genotyping analysis, etc. for special samples.

Basic Principles

The principle of HM-P16 African swine fever PCR detector: Polymerase chain reaction (PCR) is a molecular biology technology used to amplify specific DNA fragments. It can be seen as a special DNA amplification in vitro, consisting of several steps such as high-temperature denaturation, low-temperature annealing, and warm extension to form a temperature cycle, allowing the target gene to be rapidly amplified. It has strong specificity, high sensitivity, simple operation, and time-saving characteristics, and is a major innovation in gene amplification technology. PCR technology can specifically amplify extremely small amounts of target DNA by millions of times, greatly improving the analysis and detection capabilities of DNA molecules.

Real time fluorescence quantitative PCR technology is a method that adds corresponding fluorescent dyes or fluorescent labeled probes on the basis of conventional PCR. During the PCR reaction process, the entire PCR process is detected in real time through changes in fluorescence signals. The total amount of products after each polymerase chain reaction (PCR) cycle is monitored using fluorescent chemicals, and specific DNA sequences in the test sample are quantitatively analyzed using internal or external reference methods.

Basic structure

The African swine fever PCR detector is an instrument for real-time detection of reactions, mainly composed of gene amplification thermal cycle system, fluorescence real-time detection system, microcircuit control system, computer and application software. Two core functional modules are the thermal cycling system and the fluorescence real-time detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, light signal transmission components, fluorescence detection components, and control systems.

Application area

Basic scientific research

Pathogen detection

Animal epidemic detection

Meat product adulteration

□ Genetically modified testing

Food hygiene and quarantine

Drug development and rational use of drugs

Gene expression

Water monitoring

Equipment parameters