Real Time PCR System

TC1000-G

Key Features

• Reliable heating/cooling elements and precise temperature control

• Unique design of block ensures the temperature uniformity and repeatable results

• 7 inch Large color touch panel screen for easy programming with user friendly software

• High performance DSP and temperature control

• Fast heating and cooling rate

• Large user program storage

TC1000-S

Key Features

• Reliable heating/cooling elements and precise temperature control

• Unique design of block ensures the temperature uniformity and repeatable results

• 7 inch Large color touch panel screen for easy programming with user friendly software

• High performance DSP and temperature control

• Fast heating and cooling rate

• Large user program storage

1. Infectious diseases

The most valuable application area of ​​PCR in medical laboratory science is the diagnosis of infectious diseases. In theory,

as long as a sample has a pathogen present, PCR can detect it. General laboratories can also detect 10-100 gene copies,

while the current pathogen antigen detection method generally requires 105-7 pathogens to be detected. The detection of

pathogens by PCR solves the “window period” problem of immunological detection and can determine whether the disease

is in a recessive or subclinical state.Quantitative PCR research data have shown that the number of pathogens is related to the

severity,infectivity and treatment effect of infectious diseases. Many studies have shown that after human immunodeficiency

virus (HIV) infection, the length of incubation period and the severity of clinical symptoms are significantly related to the viral

load in the blood; some studies have also shown that HIV is not infectious when the viral load is below a certain value.

In the quantitative study of hepatitis B virus and hepatitis C virus, it is found that the number of viruses is related to the efficacy

of certain drugs. For example, interferon therapy is not sensitive to hepatitis virus high copies, but sensitive to low copies; while

some drugs have the effect of significantly reducing the high copies of the virus.

2. Tumor

Increased expression and mutation of oncogenes can appear in many tumors in the early and benign stages. PCR technology

can not only effectively detect gene mutations, but also accurately detect the expression of oncogenes, which can be used for

early diagnosis, classification, staging and prognosis of tumors.The formation of BCR/ABL fusion gene caused by proto-oncogene

translocation can be detected in almost all patients with chronic myeloid leukemia. Quantitative PCR technology can determine the

number of trace residual malignant cells by detecting the expression of BCR/ABL fusion gene. Efficacy of treatment and basis for

estimating the risk of recurrence.Some viral carcinogenesis is also related to viral load, and the results of FQ-PCR assay for EB viral

load have been used for early detection and follow-up of nasopharyngeal carcinoma.

3. Genetic disease

The first clinical application of PCR technology began with the detection of genetic mutations in sickle cell and beta-thalassemia.

Mutations and deletions of genes can cause imbalances in the expression of various globin genes. FQ-PCR is an effective method

for the diagnosis of thalassemia to detect the differences in the expression of various globin genes.