digisystem cubee mini cps disc centrifuge pcr plate

*The PCR consumables produced by IKEME have two capacities of  0.1ml and 0.2ml,

and the colors are transparent and white. The models are single tube, 8-tube tube,

and 12-tube tube, which are suitable for ordinary PCR and qPCR reactions.

-Key Features:-No DNase and RNase, no DNA and RNA;

-The top-level high-precision mold fully guarantees the ultra-thin and uniform tube

wall and the uniformity of the product, and has an excellent heat conduction effect,

so that the reaction liquid in the tube reaches the target temperature at the fastest speed,

and maximizes the amplification of the sample;

-The 15.4mm short tube design reduces the pollution caused by condensate, and the convex

edge design of the tapered tube effectively ensures the tightness and prevents cross-contamination;

-Made with imported high-quality materials and advanced processing technology, the flat cover has

extremely low light loss, suitable for fluorescent quantitative PCR; -No thermal analysis of the product,

no endotoxin; -Applicable to most automated laboratory instruments;

-There are directional holes for easy identification of the direction;

1. The manufacturer of PCR eight-coupled tubes talks about the operation steps

Users can choose negative pressure or centrifugation.

A. Negative pressure method

1. Correctly connect the negative pressure device, place the 96-well DNA preparation plate on the negative pressure device;

add 3 times buffer PCR-A to the PCR, enzyme digestion, enzyme labeling or sequencing reaction solution (if the buffer PCR-A

is less than 100 μl, add 100 μl); mix well and transfer to a 96-well DNA prep plate, turn on and adjust the negative pressure to -25-30 inHg,

and slowly aspirate the solution from the plate.

2. Add 0.3 ml of Buffer W2 and absorb the solution. Wash twice with 0.3 ml of buffer W2 using the same method.

Confirm addition of absolute ethanol to the indicated volume of Buffer W2 concentrate on the reagent bottle.

3. Maintain negative pressure and extract the 96-well DNA preparation plate for 10 minutes.

4. Grind the 96-well DNA preparation plate 6 times on the long fibrous tissue with the drainage tube facing down.

5. Place the 96-well DNA preparation plate on a 96-well V-shaped plate, add 25-30ul of water or elute to the center of the membrane,

and let stand for 1 minute at room temperature. DNA was eluted by centrifugation at 3 000 x g for 5 min.

B. Centrifugal

1. Add 3 volumes of Buffer PCR-A (if Buffer PCR-A is less than 100 μl, add 100 μl) to PCR, digestion, enzyme labeling or sequencing reactions;

mix and transfer to 96-well DNA in 96-well preparation plate. Place the DNA prep plate in a 96-well 1.6ml deep well plate, centrifuge

at 1000 x g for 1 min, and discard the filtrate.

2. In a 96-well DNA preparation plate, add 0.3ml of buffer W2, centrifuge at 1000×g for 1 minute, and discard the filtrate. Wash again in the

same manner with 0.3 ml of buffer W2. Confirm addition of absolute ethanol to the indicated volume of Buffer W2 concentrate on the reagent bottle.

3. Place the 96-well DNA prep plate in a 96-well 1.6ml deep-well plate and centrifuge at 3 000×g for 10 minutes.

4. Place the 96-well DNA preparation plate in a clean 96-well V-shaped bottom plate, add 25-30ul of water or elute to the center of the membrane,

and let stand for 1 minute at room temperature. DNA was eluted by centrifugation at 3 000 x g for 5 min.

2. The manufacturers of PCR eight tubes talk about the precautions:

1. Heating the eluent or water to 65°C can help improve elution efficiency.

2. DNA molecules are acidic, it is recommended to store in 2.5 mM Tris-HCl, pH 8.5 eluent.